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The present study investigated the strong impact of soil tetracycline contamination on the physiology and biochemistry of pea seedlings. Tetracycline, a widely used antibiotic in veterinary and human medicine, has been detected in soil due to its extensive usage and improper disposal. However, its effects on the growth and metabolic processes of plants remain poorly understood. In this study, pea seedlings were exposed to varying concentrations of tetracycline-contaminated soil, and their physiological and biochemical responses were assessed. The results revealed significant alterations in various parameters, including growth rate, chlorophyll content, enzymatic activities, and antioxidant defense mechanisms. These findings highlight the potential risks associated with tetracycline contamination in agricultural ecosystems and emphasize the need for further research to mitigate its adverse effects.