Zell- und Molekularbiologie

Abstrakt

Decolorization of Synthetic Textile Dye and Enzymes Production by Improved Strains of Pleurotus Species

Singh MP and Srivastava AK

In the present investigation the efficiency of three species of white rot fungui - Pleurotus and their improved dikaryons (heterokaryons) was assessed for decolorization of Phenol Red (Phenolsulfonphthalein or PSP) and production of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase enzymes. All the species of Pleurotus i.e. P. flabellatus, P. ostreatus and P. citrinopileatus decolorized PSP well. However, improved dikaryons Pfo 6X9 and Poc 9X6 decolorized the dye more effectively than three species of Pleurotus. The improved dikaryons also showed higher ligninolytic activity than the parental species. Poc 9X6 showed higher LiP (56.14 U), MnP (615.15 U) and Laccase (715.25 U) activity. In the present work different pH, age and concentration of inoculum and effect of surfactant i.e. sodium dodecyl sulphate (SDS) and Tween-80 were analyzed in order to determine the optimum ones to decolorize maximum concentration of dye. 5 ml of 10 days old culture on pH 5.5 and 0.1% Tween-80 supported maximum decolorization of PSP.