Biotechnologie und Biomaterialien

Abstrakt

Effect of Seed Treatment on Seed Germination and Seedling Growth Attributes of Yeheb (Cordeauxia edulis) with In-Vitro Conditions

Chanie Derso and Tileye Feyissa

Yeheb (Cordeauxia edulis) is an evergreen shrub in the bean family that produces seeds in semi-arid bush land on sandy soils of Somalia and Ethiopia. It is drought hardy and one of the major sources of food for both animals and humans. It also used as medicine and source of natural dyes for coloring of food and textiles and to build house as it is termite resistance. Even though it is a multipurpose plant and being encouraged by most people, it becomes endangered due to low seed viability and over exploitation. Those problems have to overcome by micro propagation and regeneration process. The pre-condition of those attempts are having adequate seed treatment and germination. Thus, the objective of this study is to know the effect of seed treatment on seed germination and seedling growth Attributes of C. edulis in In-Vitro conditions. Both coated and de-coated seeds were sterilized using different concentrations of 3%, 5%, 10% and 15% and 2-3 drops of Tween-20 for 5 min and cultured on growth regulators-free solid MS medium. Results in this study revealed that sterilization of de-coated seeds of C. edulis with 5% Clorox for 5 min was found to be valuable for both seed germination and seedling growth. As conclusion, it is important for In Vitro germinated seedlings which are essential for further experimental process of micro propagation and regeneration. Besides, it is used to as effort for further research activities by reducing time and cost.