Gemeinschaftsmedizin und Gesundheitserziehung

Abstrakt

Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

Andrea Ceja-Fdez, Ramón Carriles, Ana Lilia González-Yebra and Tzarara López-Luke

In this work, gold NPs were prepared by Turkevich method and their interaction with the HPV and cancerous cervical tissues by scanning electron microscopy, energy dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS were studied. The SEM images confirmed the presence and localization of the gold NPs inside of the two kind of tissues. The absorption light of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was due probably by the aggregation of gold NPs inside of the tissues. Therefore, the interaction of gold nanoparticles with the HPV and cancerous cervical tissues a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for rapid screening of cancer.