Zeitschrift für analytische und bioanalytische Techniken

Abstrakt

Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA)

Angel Justiz Vaillant, Norma McFarlane-Anderson, Brian Wisdom, Wayne Mohammed, Sehlule Vuma, Chalapathi Rao, Arvind Kurhade, Hellen Asemota, Shivnarine Kissoon and Geeta Kurhade

The aim of this study was to create universal chimeric conjugates able to react with both avian and mammalian immunoglobulins to be used as a reagent in Enzyme-linked Immunosorbent Assays (ELISAs). The periodate method was used in the conjugation process of linking horseradish peroxidase to immunoglobulin-binding bacterial proteins. ELISAs were used to prove the efficacy of the conjugates, namely newly synthesize conjugates (NSC) in the detection of immunoglobulins. All NSC bound to mammalian immunoglobulins, but failed to bind avian immunoglobulin Y (IgY), with the exception of the SpLAG-anti-IgY-HRP that was the most versatile binding to the all panel of purified immunoglobulin and sera. In conclusion, the results of these experiments show that SpLAG-anti-IgY-HRP conjugate, a novel reagent, was the most versatile product among NSC and commercially-available conjugates.