Biotechnologie und Biomaterialien

Abstrakt

Production of Proteases by Genetically Improved Bacillus subtilis for Enhanced Skin Penetration of Antibacterial Topical Formulation

Faisal Shahzad, Sohail Ahmad, Habib Ahmad, Qazi Abdur Rashid, Nadia Parveen, Fatima Javed, Rida Khan, Muhammad Zeeshan Ashraf and Faiza Naseer

The present study was designed for the production, isolation and extraction of protease enzyme from Bacillus subtilis and to investigate the role of protease enzyme mixed with standard antibacterial formulation, i.e. Silver Sulphadiazine 1% w/w cream in burnt abscessed wounds to improve and speed up the healing process. 18 male rabbits weighing between 1.8-2 kg were divided into 3 groups. A burnt abscessed wound model was created on the back of all the rabbits and pathogenic Streptococci introduced into the wound. Then the burnt abscessed areas in 1st, 2nd and 3rd groups were applied daily with cold cream (control), Silver Sulphadiazine 1% w/w cream and Silver Sulphadiazine 1% + Protease w/w cream respectively. The wounds were examined on a daily basis for the healing process; sizes were measured and recorded in the form of photographs on 2nd, 5th, 10th and 14th day. The rabbits were anesthetized and skin tissue samples of each group were collected for histo-pathological examinations. Significant improvement in wound healing with Silver Sulfadiazine 1% w/w and Proteases cream was noticed as compared to Silver Sulphadiazine 1% w/w cream and cold cream (control). It was concluded that application of Silver Sulfadiazine 1% w/w and Proteases was appreciably useful in healing of burnt and abscessed skin wounds in animal model (rabbit) and antibacterial formulation Silver Sulphadiazine mixed protease enzyme can be exploited in pharmaceutical field as an effective promoter of wound healing. The studies can be extended further for formulation stability and clinical trials on human.