Internationale Zeitschrift für Forschung und Entwicklung in Pharmazie und Biowissenschaften

Abstrakt

SCREENING OF ANTIBACTERIAL ACTIVITY AND PHYTO-COMPOUND STUDIES OF Aduthodavasicaneesvasicanees

J. Rosaline Vimalaand, P. Angel Evanjalin

Medicinal plants contribute in human health care system. Most of the plants utilized by village people are folk medicine. Now we are turned into medicinal plant analysis of active compounds and conservation aspect. The medicinal plants are used in traditional treatments to cure variety of diseases for thousands of year. The aim of this study is to identify such plants with antibacterial efficacy for controlling human pathogens.The present study describes the phytochemical profile and antimicrobial activity ofAduthodavasicaneesvasicanees. For the present investigation the ethanol extract of the leaves of Adutodavasicanees were tested for their antimicrobial activity and further subjected to qualitative analysis and GC-MS analysis to find out the nature of the compounds responsible for the antimicrobial activity. The anti-bacterial and anti-fungal activities were assessed by measuring the diameter of the inhibition zones of the extract against the bacterial strains such as Escherichia coli,Salmonella paratyphi, Vibrio cholera, and Staphylococcusaureus andfungal pathogens namely Aspergillusniger, Aspergillusfumigatus, Candida albicans. The extract has a marked sensitivity towards Staphylococcus aureus, with 28.0 mm at concentration 1000 μg/ml respectively against the standard which has 25.0 mm of inhibition efficiency and the extract has a marked sensitivity towards Candida albicans, with 19.0 mm at concentration 1000 μg/ml respectively against the standard which has 26.0 mm of inhibition efficiency at a concentration of 50 mg/ml. The phytochemical screening revealed the presence of various secondary metabolites such as alkaloids, carbohydrates, pseudo tannins, chlorogenic acids, steroidal glycosides, saponins and flavonoids . GC-MS analysis revealed that the ethanol extract contains 1,6,10-Dodecatriene,7,11-dimethyl-3-methylene-,(E)- (20.64%), Trifluoroacetyllavandulol (6.18), 2,3-Diamino-2-cyanosuccinonitrile (2.58), Tetrazolo[1,5-b]1,2,4-triazine,5,6,7,8-tetrahydro-, 7-dimethyl-(3.28%), Propanoic acid, 2-methyl-, 2- ethyl-1-propyl-1,3- propanediyl ester(67.24%), 3-Butyn-2-ol(0.08%) to be the molecules responsible for the antimicrobial activity of Aduthodavasicaneesvasicanees. It was concluded that plant extract can be used as a preservatives against the human pathogens.