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Short Note on Normal-Phase Chiral Chromatography

Amane Santdos Ummara

Normal-phase chiral chromatography is a powerful analytical technique used for the separation and analysis of enantiomers based on their interaction with chiral stationary phases. Enantiomers are mirror-image isomers that possess identical physical and chemical properties but exhibit distinct biological activities. Normal-phase chiral chromatography operates on the principle of differential adsorption or partitioning of enantiomers onto a polar chiral stationary phase. The more polar enantiomer has stronger interactions with the stationary phase, leading to slower elution and longer retention time. This technique offers high resolution and selectivity, making it suitable for the separation of polar and moderately polar compounds. Normal-phase chiral chromatography finds applications in various industries, including pharmaceuticals, agrochemicals, and fine chemicals. Advancements in this field include the development of new chiral stationary phases and the use of chiral additives or modifiers in the mobile phase to enhance separation efficiency. Overall, normal-phase chiral chromatography provides valuable insights into the stereochemistry of chiral compounds and is widely utilized for enantiomeric analysis and purification.