Internationale Zeitschrift für Forschung und Entwicklung in Pharmazie und Biowissenschaften

Abstrakt

WOUND HEALING ACTIVITY FROM THE LEAF EXTRACTS OF MORUS LAEVIGATA AND IN SILICO BINDING STUDIES FROM ITS ISOLATES WITH GSK 3-�²

Aditya Rao SJ, Jeevitha B, Smitha R, Ramesh CK, Paramesha M, Jamuna KS

Background: Morus plant species possess enormous importance in the field of medicine and Morus laevigata, a white variety of genus Morus, is a popular hybrid species which is highly appreciated for its medicinal properties.
Methods: The present investigation is aimed to study the effect on wound healing capacity of different solvent extracts of Morus laevigata. Further, In-silico binding studies were carried out for a different drug candidates isolated from Morus laevigata taking nitrofurazone as a standard reference and CHIR-98014 as a positive control against glycogen synthase kinase 3-β (GSK3- β) protein.
Results: The results of the wound healing studies revealed that the methanolic extract has better wound healing activity in terms of mean time of epithelization (18.53±0.33 days) and wound breaking strength (1258.33±59.74) when compared to nitrofurazone (16.36±0.08 days and 1683.33±20.28). Further, the results of the virtual screening revealed that among the three molecules tested, morusin showed minimum binding (-9.47 kJ mol-1) and docking energy (-11.35 kJ mol-1) when compared with the standard reference nitrofurazone (-6.03 kJ mol-1 and -6.29 kJ mol-1).
Conclusion: the active constituents isolated from Morus laevigata supports the wound healing ability of the different solvent extracts in terms of different binding models.