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Emam Ahmed MM, Haque MM, Arif Hasan Khan Robin ABM, Hossain MA and Biswash MR
The experiment was conducted to study the early establishment osmotic stress of advanced deep water rice. Severe parameters such as percent germination, seedling height, and number of leaves, leaf length, node length, and root length were studied for in vitro study. This study was conducted by MS medium supplemented with two treatments of PEG (1% and 5% PEG) and control (0% PEG). For percent germination, Bazail, HBJ.A.IV, and Birpala showed better performance with control (0%) but Gabura and Lalkhama performed better in both T1 (1% PEG) and T2 ( 5% PEG). The genotype Gabura showed more seedling height under control and HBJ.A.IV with T1 and T2. At 5% PEG, more number of leaves was found in BR5915-B-7, Gabura and Lalkhama. HBJ.A.IV and Lalkhama showed more leaf length and node length at T2. The genotype BR224-2B-2-5 was best and control and T1. But Birpala showed more root length at T2. The information generated in this study indicated that there is a close relationship between in vitro studies for the growth performance of the rice genotypes although significant variation was observed within the genotypes. Finally, the advanced genotype BR224-2B-2-5 may be a good source to meet the future challenge.