Zeitschrift für Zelluläre und Molekulare Pharmakologie

Abstrakt

Effects and Mechanisms of Xiaochaihu Tang against Liver Fibrosis: An Integration of Network Medicine, Molecular Moorage and Experimental Validation

Sai Krishna

Ethno pharmacological connectedness: Liver pathology could be a probably harmful chronic disease caused by numerous etiologies. there’s presently no specific drug for liver pathology. Xiaochaihu Tang (XCHT) could be a ancient formula combined of seven herbs, that was 1st recorded within the writing on symptom Diseases in Han dynasty of ancient China. it’s wide utilized in clinic to internal organ protection, analgesic, antipyretic and anti inflammatory treatment. And it’s been suggested for treating chronic infectious disease and chronic rubor within the latest tips for the designation and treatment of liver pathology with integrated ancient and western drugs. However, the underlying restrictive mechanisms stay elusive.

Aim of the study: This study aims to explore the therapeutic effects of XCHT on liver pathology and its underlying molecular mechanisms from the attitude of network medicine and experimental analysis.

Materials and strategies: Carbon tetrachloride (CCl4) iatrogenic and channel tying (BDL) iatrogenic liver pathology models in mice were established to guage the anti-fibrosis effects of XCHT in vivo. Potential antifibrosis targets of XCHT were screened via network institution [1]. Through GO and route enrichment analysis, the underlying mechanisms were discovered.Then, the core targets were known from protein-protein interaction network by means that of the Cytohubba plug-in of Cytoscape. what is more, 2 effective compound parts of XCHT were recognized by molecular moorage. Moreover, the anticipated parts and pathways were verified by in vitro experiments [2].

Results: When treated with XCHT, liver pathology was mitigated in each mice models, showing because the improvement of liver perform, the protection of hepatocytes, the inhibition of HSC activation and also the reduction of internal organ scleroprotein accumulation. 540 compound parts, three hundred therapeutic targets, 109 communication pathways, 246 GO biological processes, seventy seven GO cellular parts, 107 GO molecular functions things and core targets were known by network analysis[3]. Then, 6-gingerol and baicalein were known because the core parts of anti-fibrosis effects of XCHT via leptin or Nrf2 communication pathway. what is more, the experiment in vitro conjointly valid the results.

Conclusions: Our study suggests XCHT might alleviate liver pathology through multi-targets and multipathways; 6-gingerol and baicalein ar its core parts which can play a crucial role via leptin or Nrf2 communication pathway [4].