Zeitschrift für klinische und experimentelle Pathologie

Abstrakt

Prolactin, Thyroid Stimulating Hormone and Thyroid Hormones (Ft3, Ft4) Concentrations in Female Patients with Inferility: An Observational Study

Madeeha Subhan, Waleed Sadiq, Sanniyah Subhan

Objective: To determine the variations in thyroid hormones, thyroid stimulating hormone and prolactin levels in females of our population.

Materials and methods:

Subject characteristics: Patients aged 13 to 60 years were studied for their hormonal profile. The inclusion criteria had patients with complaints of mild reproductive and thyroid dysfunction. Patients with severe disorders and cancerous conditions were excluded from the study.

Blood sampling: 3 ml blood was drawn and transferred to clean test tubes. Blood was centrifuged at 3000 rpm for 10-15 min. Serum was separated and stored at -20°C.

Hormonal assay: Thyroid stimulating hormone (TSH), Prolactin, FT3 and FT4 were assayed in the serum. Equipment used in laboratory was Eclecsys 2010 (Roche). Principle of the hormonal test was based on ECL (Electrochemiluminescence). Standard kits by Roche were used for chemical analysis. The results were analyzed by using ANOVA in SPSS (version 10.0 for windows).

Results: A total of 97 patients were tested. The patients were divided into four major groups, showed overall nonsignificant variation in hormonal level for Prolactin, FT3 and FT4 (p>0.05). About 80% patients were found normal, in TSH estimation. Women from age 16-35 years old were greater in number than any other age group. Means for each hormone were non-significant at p=0.05.

Conclusion: Hyperprolactinemia causes reproductive disorders in women, early diagnosis and treatment of this disease is important. In conclusion estimation of prolactin and TSH levels might be considered essential in assessment of patient with fertility disorders.